Hsp90 stabilizes Cdc25A and counteracts heat shock-mediated Cdc25A degradation and cell-cycle attenuation in pancreatic carcinoma cells

作者：Giessrigl Benedikt; Krieger Sigurd; Rosner Margit; Huttary Nicole; Saiko Philipp; Alami Mouad; Messaoudi Samir; Peyrat Jean Francois; Maciuk Alexandre; Gollinger Michaela; Kopf Sabine; Kazlauskas Egidijus; Mazal Peter; Szekeres Thomas; Hengstschlaeger Markus; Matulis Daumantas; Jaeger Walter; Krupitza Georg^*

来源：Human Molecular Genetics, 2012, 21(21): 4615-4627.

DOI：10.1093/hmg/dds303

Pancreas cancer cells escape most treatment options. Heat shock protein (Hsp)90 is frequently over-expressed in pancreas carcinomas and protects a number of cell-cycle regulators such as the proto-oncogene Cdc25A. We show that inhibition of Hsp90 with geldanamycin (GD) destabilizes Cdc25A independent of Chk1/2, whereas the standard drug for pancreas carcinoma treatment, gemcitabine (GEM), causes Cdc25A degradation through the activation of Chk2. Both agents applied together additively inhibit the expression of Cdc25A and the proliferation of pancreas carcinoma cells thereby demonstrating that both Cdc25A-destabilizing/degrading pathways are separated. The role of Hsp90 as stabilizer of Cdc25A in pancreas carcinoma cells is further supported by two novel synthetic inhibitors 4-tosylcyclonovobiocic acid and 7-tosylcyclonovobiocic acid and specific Hsp90AB1 (Hsp90) shRNA. Our data show that targeting Hsp90 reduced the resistance of pancreas carcinoma cells to treatment with GEM.

• 出版日期2012-11-1
• 单位河北医科大学