A general strategy for performing temperature-programming in high performance liquid chromatography-Further improvements in the accuracy of retention time predictions of segmented temperature gradients

作者：Wiese Steffen; Teutenberg Thorsten^*; Schmidt Torsten C

来源：Journal of Chromatography A, 2012, 1222: 71-80.

DOI：10.1016/j.chroma.2011.12.018

• Temperature-programming
• High-temperature high performance liquid chromatography (IT-HPLC)
• Heating system
• Method development
• Linear elution strength model (LES)
• Linear solvent strength model (LSS)

In the present work it is shown that the linear elution strength (LES) model which was adapted from temperature-programming gas chromatography (CC) can also be employed for systematic method development in high-temperature liquid chromatography (HT-HPLC). The ability to predict isothermal retention times based on temperature-gradient as well as isothermal input data was investigated. For a small temperature interval of Delta T = 40 degrees C, both approaches result in very similar predictions. Average relative errors of predicted retention times of 2.7% and 1.9% were observed for simulations based on isothermal and temperature-gradient measurements, respectively. Concurrently, it was investigated whether the accuracy of retention time predictions of segmented temperature gradients can be further improved by temperature dependent calculation of the parameter S-T of the LES relationship. It was found that the accuracy of retention time predictions of multi-step temperature gradients can be improved to around 1.5%, if S-T was also calculated temperature dependent. The adjusted experimental design making use of four temperature-gradient measurements was applied for systematic method development of selected food additives by high-temperature liquid chromatography. Method development was performed within a temperature interval from 40 degrees C to 180 degrees C using water as mobile phase. Two separation methods were established where selected food additives were baseline separated. In addition, a good agreement between simulation and experiment was observed, because an average relative error of predicted retention times of complex segmented temperature gradients less than 5% was observed. Finally, a schedule of recommendations to assist the practitioner during systematic method development in high-temperature liquid chromatography was established.

• 出版日期2012-1-27