Alternative Formation of Red-Shifted Channelrhodopsins: Noncovalent Incorporation with Retinal-Based Enamine-Type Schiff Bases and Mutated Channelopsin

作者：Okitsu Takashi; Matsuyama Take; Yamashita Takahiro; Ishizuka Toru; Yawo Hiromu; Imamoto Yasushi; Shichida Yoshinori; Wada Akimori^*

来源：Chemical & Pharmaceutical Bulletin, 2017, 65(4): 356-358.

DOI：10.1248/cpb.c17-00054

• channelrhodopsin
• chromophore
• red-shift
• neuroscience
• optogenetics

Red-shifted channelrhodopsins (ChRs) are attractive for optogenetic tools. We developed a new type of red-shifted ChRs that utilized noncovalent incorporation of retinal and 3,4-dehydroretinal-based enamine-type Schiff bases and mutated channelopsin, C1C2-K296G. These ChRs exhibited absorption maxima that were shifted 10-30nm toward longer wavelengths than that of C1C2-ChR regenerated with all trans-retinal.

• 出版日期2017-4
• 单位RIKEN