Diagnostic model of saliva protein finger print analysis of patients with gastric cancer

作者：Wu, Zheng Zhi^*; Wang, Ji Guo; Zhang, Xiao Li

来源：World Journal of Gastroenterology, 2009, 15(7): 865-870.

DOI：10.3748/wjg.15.865

• Saliva
• Protein finger print model
• Gastric cancer
• Matrix-assisted laser desorption ionization-time-of-flight/mass spectrometry
• Weak cation exchange
• Magnetic bead
• Proteomics

AIM: To explore the method for early diagnosis of gastric cancer by screening the expression spectrum of saliva protein in gastric cancer patients using mass spectrometry for proteomics.
METHODS: Proportional peptide mass fingerprints were obtained by analysis based on proteomics matrix-assisted laser desorption ionization time-of-flight/mass spectrometry. A diagnosis model was established using weak cation exchange magnetic beads to test saliva specimens from gastric cancer patients and healthy subjects.
RESULTS: Significant differences were observed in the mass to charge ratio (m/z) peaks of four proteins (1472.78 Da, 2936.49 Da, 6556.81 Da and 7081.17 Da) between gastric cancer patients and healthy subjects.
CONCLUSION: The finger print mass spectrum of saliva protein in patients with gastric cancer can be established using gastric cancer proteomics. A diagnostic model for distinguishing protein expression mass spectra of gastric cancer from non-gastric-cancer saliva can be established according to the different expression of proteins 1472.78 Da, 2936.49 Da, 6556.81 Da and 7081.17 Da. The method forearly diagnosis of gastric cancer is of certain value for screening special biological markers.

• 出版日期2009-2-21
• 单位南方医科大学