Molecular cloning and antiviral activity of IFN-beta promoter stimulator-1 (IPS-1) gene in Japanese flounder, Paralichthys olivaceus

作者：Simora Rhoda Mae C; Ohtani Maki; Hikima Jun ichi; Kondo Hidehiro; Hirono Ikuo; Jung Tae Sung; Aoki Takashi^*

来源：Fish & Shellfish Immunology, 2010, 29(6): 979-986.

DOI：10.1016/j.fsi.2010.08.012

• Japanese flounder
• Mitochondrial adaptor
• IPS-1 (MAVS)
• HIRRV
• VHSV

The mitochondrial adaptor, IFN-beta promoter stimulator-1 (IPS-1), also known as MAVS/VISA/Cardif, plays a key role in the signal transduction of the RIG-1/MDA5 pathway to induce the production of interferons (IFNs) and other cytokines. In the present study, Japanese flounder (Paralichthys olivaceus) IPS-1 cDNA was cloned from Japanese flounder spleen using PCR-based methods. The full-length cDNA has 2235 nucleotides and encodes a polypeptide of 641 amino acids. The putative Japanese flounder IPS-1 protein contains an N-terminal CARD-like domain, a central proline-rich domain, a C-terminal transmembrane domain, and exhibits similarity to other teleost counterparts ranging from 20% to 34%. Semi-quantitative RT-PCR showed that Japanese flounder IPS-1 mRNA was expressed in all tissues examined. The expression level of flounder IPS-1 gene was unchanged in viral hemorrhagic septicemia virus (VHSV)-infected kidney as measured by quantitative real-time PCR (Q-PCR). In addition, Japanese flounder IPS-1-overexpressing cells were protected against hirame rhabdovirus (HIRRV) and VHSV infection as manifested by the delayed appearance of cytopathic effect (CPE) and decreased viral titers. Expression of IFN-inducible genes including Mx, ISG15 and IRF3 were also induced in the IPS-1-overexpressing cells. These results suggest that Japanese flounder IPS-1 is involved in the antiviral immunity as a one of the adaptors in fish IFN-activation pathway.

• 出版日期2010-12