Adult human circulating CD34(-)Lin(-)CD45(-)CD133(-) cells can differentiate into hematopoietic and endothelial cells

作者：Ciraci Elisa; Della Bella Silvia; Salvucci Ombretta; Rofani Cristina; Segarra Marta; Bason Caterina; Molinari Agnese; Maric Dragan; Tosato Giovanna; Berardi Anna C^*

来源：Blood, 2011, 118(8): 2105-2115.

DOI：10.1182/blood-2010-10-316596

A precise identification of adult human hemangioblast is still lacking. To identify circulating precursors having the developmental potential of the hemangioblast, we established a new ex vivo long-term culture model supporting the differentiation of both hematopoietic and endothelial cell lineages. We identified from peripheral blood a population lacking the expression of CD34, lineage markers, CD45 and CD133 (CD34(-)Lin(-)CD45(-)CD133(-) cells), endowed with the ability to differentiate after a 6-week culture into both hematopoietic and endothelial lineages. The bilineage potential of CD34(-)Lin(-)CD45(-)CD133(-) cells was determined at the single-cell level in vitro and was confirmed by transplantation into NOD/SCID mice. In vivo, CD34(-)Lin(-)CD45(-)CD133(-) cells showed the ability to reconstitute hematopoietic tissue and to generate functional endothelial cells that contribute to new vessel formation during tumor angiogenesis. Molecular characterization of CD34(-)Lin(-)CD45(-)CD133(-) cells unveiled a stem cell profile compatible with both hematopoietic and endothelial potentials, characterized by the expression of c-Kit and CXCR4 as well as EphB4, EphB2, and ephrinB2. Further molecular and functional characterization of CD34(-)Lin(-)CD45(-)CD133(-) cells will help dissect their physiologic role in blood and blood vessel maintenance and repair in adult life. (Blood. 2011;118(8):2105-2115)

• 出版日期2011-8-25