Azadirachta indica mitigates behavioral impairments, oxidative damage, histological alterations and apoptosis in focal cerebral ischemia-reperfusion model of rats

作者：Vaibhav Kumar; Shrivastava Pallavi; Khan Andleeb; Javed Hayate; Tabassum Rizwana; Ahmed Md Ejaz; Khan M Badruzzaman; Khan Mohd Moshahid; Islam Farah; Ahmad Sayeed; Siddiqui M Saeed; Safhi Mohammed M; Islam Fakhrul

来源：Neurological Sciences, 2013, 34(8): 1321-1330.

DOI：10.1007/s10072-012-1238-z

• Antioxidant
• Azadirachta indica
• Behavioral deficit
• Caspase-3
• Oxidative stress

Azadirachta indica Linn. (Meliaceae) has been used from ancient times as a remedy for various ailments. The present study was designed to investigate the antioxidant and anti-apoptotic properties of A. indica seed extract (ASE) in transient middle cerebral artery occlusion (MCAO) rat model. Antioxidant potential of ASE was determined in vitro. Further, ASE was evaluated against neurological deficits, histological alterations (TTC, CV and H&E) and oxidative damage (TBARS, GSH and nitrite) in MCAO rats. Moreover, caspase-3 and -9 were analyzed to evaluate the anti-apoptotic activity of ASE. ASE has shown potent in vitro reducing power (126.2 mg AsAE/g extract) and free radical scavenging activities (DPPH 171.0 and NO 176.0 mu g/ml). Furthermore, ASE inhibited oxidative stress and decreased the activities of caspase-3 (26.7 %, p < 0.05) and caspase-9 (31.2 %, p < 0.01) thus, reduced neuronal loss in MCAO rats. Our data revealed that ASE has potent antioxidant and anti-apoptotic properties, and may be explored for its active constituents against neurodegenerative diseases.

• 出版日期2013-8