A reduction of viral mRNA, proteins and induction of altered morphogenesis reveals the anti-HTLV-1 activity of the labdane-diterpene myriadenolide in vitro

作者：Silveira Martins Camila Pacheco; Gomes Orlando Abreu; Martins Marina Lobato; de Carvalho Luciana Debortoli; de Souza Jaqueline Gontijo; Da Fonseca Flavio Guimaraes; Silva dos Santos Rodrigo Gonalves; Andrade Margareth Spangler; Zani Carlos Leomar; de Souza Fagundes Elaine Maria; Barbosa Stancioli Edel Figueiredo^*

来源：BMC Microbiology, 2014, 14(1): 331.

DOI：10.1186/s12866-014-0331-2

• HTLV-1
• Diterpene myriadenolide
• Antiviral activity
• HAM/TSP
• ATL

Background: Human T-lymphotropic virus 1 (HTLV-1) has been associated with leukemia/lymphoma (ATL) and myelopathy/tropical spastic paraparesis (HAM/TSP), in addition to other inflammatory diseases as well as infection complications. Therapeutic approaches for HTLV-1-related pathologies are limited. The labdane diterpene myriadenolide (AMY) is a natural product that exhibit biological activities, such as anti-inflammatory and antiviral activity as reported for HIV and herpesvirus. %26lt;br%26gt;Results: We demonstrated that this natural product was able to inhibit the expression of gag-pol mRNA and substantially reduced the expression of the structural proteins p19 and gp46. Comparison of treated and untreated cells shows that AMY alters both the morphology and the release of viral particles. The Atomic Force Microscopy assay showed that the AMY treatment reduced the number of particles on the cell surface by 47%. %26lt;br%26gt;Conclusion: We demonstrated that the labdane diterpene myriadenolide reduced the expression of the structural proteins and the budding of viral particles, besides induces altered morphogenesis of HTLV-1, conferring on AMY a new antiviral activity that may be useful for the development of new compounds with specific anti-HTLV-1 activity.

• 出版日期2014-12-24