Antibacterial activity of cationised and non-cationised placebo lipidic nanoemulsion using transmission electron microscopy

作者：Singh Neeru^*; Verma Saurabh Manaswita; Singh Sandeep Kumar; Verma Priya Ranjan Prasad; Ahsan Mohd Neyaz

来源：Journal of Experimental Nanoscience, 2015, 10(4): 299-309.

DOI：10.1080/17458080.2013.830199

• transmission electron microscopy
• cationic lipids
• photon correlation spectroscopy
• Escherichia coli

The primary aim of the study was to observe the morphological changes taking place on Escherichia coli when treated with non-cationised placebo lipidic emulsion (NCPLE) and cationised placebo lipidic emulsion (CPLE). The particle size of NCPLE and CPLE indicated the mean diameter of 10.77 +/- 0.20nm and 56.98 +/- 1.718nm, respectively. The zeta potential of NCPLE and CPLE depicted the potential of -10.8 +/- 0.80mV and +20.9 +/- 3.25mV, respectively. Agar diffusion study indicated higher inhibition zone of 21.4 +/- 0.85mm for CPLE as evaluated against 14.7 +/- 0.76mm for NCPLE. Transmission electron micrographs of Escherichia coli treated with NCPLE and CPLE showed the potential of blank nanoemulsions as antibacterial, which can be used as a therapeutic agent. The bacterial cell being negatively charged attracts the cationised nanoemulsions from the bulk of dispersion. As the concentration of nanoemulsion increased in the vicinity of the bacterial cell, the degree of collisions increased between the cationised nanoemulsion leading to coalescence and the formation of a pool of emulsion containing bacterial cells leading to cell death. The study demonstrated that placebo lipidic nanoemulsion (NCPLE & CPLE) may itself be used as an active therapeutic agent for the treatment of bacterial infections. Moreover, it can also be used as a carrier system for antibacterial drugs for the two-pronged synergistic effect.

• 出版日期2015-3-4