Novel Luminescent Probe Based on a Terbium(III) Complex for Hemoglobin Determination

作者：Yegorova A V^*; Leonenko I I; Aleksandrova D I; Scrypynets Yu V; Antonovich V P; Ukrainets I V

来源：Journal of Applied Spectroscopy, 2014, 81(4): 672-677.

DOI：10.1007/s10812-014-9988-4

• hemoglobin
• terbium complexes
• luminescence

We have studied the spectral luminescent properties of Tb(III) and Eu(III) complexes with a number of novel derivatives of oxoquinoline-3-carboxylic acid amides (L-1-L-5 ). We have observed quenching of the luminescence of 1:1 Tb(III)-L1-5 complexes by hemoglobin (Hb), which is explained by resonance energy transfer of electronic excitation from the donor (Tb(III)-L1-5 ) to the acceptor (Hb). Using the novel luminescent probe Tb(III)-L-1, we have developed a method for determining Hb in human blood. The calibration Stern-Volmer plot is linear in the Hb concentration range 0.6-36.0 mu g/mL, detection limit 0.2 mu g/mL (3 center dot 10(-9) mol/L).

• 出版日期2014-9