Use of attenuated total reflectance Fourier transform infrared spectroscopy to monitor the development of lipid aggregate structures

作者：Hernandez Mateo R; Towns Elyse N; Ng Terry C; Walsh Brian C; Osibanjo Richard; Parikh Atul N; Land Donald P^*

来源：Applied Optics, 2012, 51(15): 2842-2846.

DOI：10.1364/AO.51.002842

Attenuated total reflectance Fourier transform infrared spectroscopy is used to monitor the adsorption of 100 nm 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) phospholipid vesicles to the surfaces of Ge, electrolessly deposited Au, and a well formed self-assembled monolayer of 1-octadecanethiol. The interaction of DPPC vesicles in solution with these different surfaces yields distinctly different surface structures: intact DPPC vesicles on Ge, a supported phospholipid bilayer on an electrolessly deposited Au surface, and a phospholipid monolayer onto the hydrophobic self-assembled monolayer. IR peak position, bandwidth, and intensity are used to confirm structure formation and quantitation of the amount of lipid that desorbs during film formation.

• 出版日期2012-5-20