Multiplexed 3D cellular super-resolution imaging with DNA-PAINT and Exchange-PAINT

作者：Jungmann, Ralf; Avendano, Maier S.; Woehrstein, Johannes B.; Dai, Mingjie; Shih, William M.; Yin, Peng^*

来源：Nature Methods, 2014, 11(3): 313-U292.

DOI：10.1038/NMETH.2835

• OPTICAL RECONSTRUCTION MICROSCOPY
• ORIGAMI
• BINDING
• CELLS
• SHAPES
• SYSTEM
• PROBES
• STORM
• LIMIT
• DYES

Super-resolution fluorescence microscopy is a powerful tool for biological research, but obtaining multiplexed images for a large number of distinct target species remains challenging. Here we use the transient binding of short fluorescently labeled oligonucleotides (DNA-PAINT, a variation of point accumulation for imaging in nanoscale topography) for simple and easy-to-implement multiplexed super-resolution imaging that achieves sub-10-nm spatial resolution in vitro on synthetic DNA structures. We also report a multiplexing approach (Exchange-PAINT) that allows sequential imaging of multiple targets using only a single dye and a single laser source. We experimentally demonstrate ten-color super-resolution imaging in vitro on synthetic DNA structures as well as four-color two-dimensional (2D) imaging and three-color 3D imaging of proteins in fixed cells.

• 出版日期2014-3