Genetic Structure of an Endangered Cycas Revoluta using RAPD Markers

作者：Indira Sarethy P; Natasha Gulati; Akanksha Bansal; Vibhuti Gupta; Kunal Malhotra; Reema Gabrani

来源：Research Journal of Biotechnology, 2011, 6(1): 50-55.

• Genetic diversity
• Cycas revolute
• RAPD
• AMOVA
• PCO
• India

Cycads are one of the most primitive species and represent a key point in evolution between ferns and angiosperms. Cycas revoluta is an introduced species in India, widely exploited for its ornamental and medicinal features, facing extensive habitat destruction. Eight accessions were collected from three regions. These were considered to belong to three populations assuming each population to be homogeneous. Genetic diversity within and amongst accessions was studied using fifteen RAPD primers. Multiple bands of scorable intensity were reproducibly generated from 10 decamers. A total of 51 loci (82.26%) were found to be polymorphic. Co-efficient of gene differentiation (G(ST)) was 0.4260, indicating that 57.4% of variation existed within populations. To remove the upward bias of G(ST), AMOVA analysis was used to confirm that a non-significant amount of genetic diversity (25%) was attributable to variance between populations while a larger share (75%) of the diversity occurred within populations. The principal co-ordinates analysis also confirmed AMOVA results. Since our sampling was from locations widely separated (2000 km), this level of genetic diversity could be because of genetic drift or ancestral polymorphisms. Based on our results and the high level of genetic diversity observed, conservation strategies should be formulated focusing on collection of samples from as many locations as possible.

• 出版日期2011-2