Adhesive cell cultivation on polymer particle having grafted epoxy polymer chain

作者：Yasuda Masahiro^*; Kunieda Hiroshi; Ono Kentaro; Ogino Hiroyasu; Iwasaki Tomohiro; Hiramoto Masaki; Glomm Wilhelm Robert; Hirabayashi Yukio; Aizawa Shin

来源：Tissue and Cell, 2011, 43(2): 115-124.

DOI：10.1016/j.tice.2010.12.007

• Graft polymer chain
• Poly(glycidyl methacrylate)
• Suspension polymerization
• Adhesive cell
• Co-culture
• Extracellular matrix

In this study, we synthesized a new cell immobilization support having poly(glycidyl methacrylate) as a graft polymer chain and used this support for cell cultivation. Base polymer particle was synthesized by suspension polymerization and epoxy polymer chain was extended from particle surface on graft polymerization. Produced polymer particles had broad particle size distribution ranging from 20 to 1000 mu m and the degree of polymerization of grafted polymer chain was ranged from 500 to 1000. The effects of various factors, such as grafted polymer chain length and its surface density, composition of base polymer network and graft polymer chain, on the cell growth of murine fibroblast cell line (MS-5 cell) on polymer particle were studied. This polymer particle could cultivate not only fibroblast cell line but also epidermal cell line (HeLa cell), osteoblast cell line (MC3T3E1 cell), and chondrocyte cell line (ch-8 cell) on its surface. Growth rate is almost the same as that of cells using poly(styrene) tissue culture dish. To apply this cell cultivation system for examination of cell co-culture, HeLa cell immobilized on 100 mu m of polymer particle was successfully co-cultured with MS-5 cell immobilized on 300 mu m of polymer particle for four weeks.

• 出版日期2011-4