Anti-inflammatory Activity of Guluronate Oligosaccharides Obtained by Oxidative Degradation from Alginate in Lipopolysaccharide-Activated Murine Macrophage RAW 264.7 Cells

作者：Zhou, Rui; Shi, Xuyang; Gao, Yan; Cai, Nan; Jiang, Zedong; Xu, Xu^*

来源：Journal of Agricultural and Food Chemistry, 2015, 63(1): 160-168.

DOI：10.1021/jf503548a

• alginate oligosaccharides
• lipopolysaccharide
• Toll-like receptor 4
• nuclear factor-kappa B
• mitogen activated protein kinases

Alginate has notably diverse pharmacological activities. The present study investigated the anti-inflammatory activity of the guluronate oligosaccharides prepared by oxidative degradation (GOS-OD) from alginate. GOS-OD significantly attenuated the production of nitric oxide (NO), prostaglandin E2 (PGE2), and reactive oxygen species (ROS), the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, and the secretion of pro-inflammatory cytokines in lipopolysaccharide (LPS)-activated murine macrophage RAW 264.7 cells. Moreover, GOS-OD potently decreased the binding of LPS to the cell surface and LPS-induced Toll-like receptor 4 (TLR4) and cluster of differentiation (CD) 14 expression. Additionally, GOS-OD could remarkably inhibit the LPS-induced activation of nuclear factor (NF)-kappa B and mitogen-activated protein (MAP) kinase pathways in RAW 264.7 cells. These results indicate that GOS-OD may reduce the LPS-stimulated inflammatory responses through blocking the activation of NF-kappa B and MAP kinases, suggesting that GOS-OD may be considered as a potential nutraceutical for inflammation.

• 出版日期2015-1-14
• 单位深圳大学