Recovery of Mycoplasma agalactiae from the ears of goats experimentally infected by the intramammary route

作者：de la Fe Christian^*; Castro Alonso A; Herraez P; Poveda Jose B

来源：Veterinary Journal, 2011, 190(1): 94-97.

DOI：10.1016/j.tvjl.2010.09.010

• Mycoplasma agalactiae
• Goats
• Intramammary inoculation
• Ear colonisation

The role of inapparent carriers of Mycoplasma agalactiae and the strategies used to colonise the external ear canal in goats remain unclear. This study examined the ability of M. agalactiae to colonise the ears of goats infected experimentally by the intramammary route. The right mammary glands of 15 lactating goats were inoculated with 10(10) colony forming units (cfu) of M. agalactiae. The goats were randomly assigned to three groups of five animals each and sampled at slaughter at 5, 15 or 45 days post-infection (dpi). A further four goats served as uninfected controls. Right and left ear swabs were collected for detection of M. agalactiae by culture before and after sacrifice. M. agalactiae was detected in 19/20 (95%) ear swabs from goats sampled at 15 and 45 dpi, whereas all ear swabs collected before inoculation, ear swabs collected from the group sampled at 5 dpi and ear swabs from control goats at the time of sacrifice were negative for M. agalactiae. Blood samples collected at 6, 12, 24, 48 and 72 h post-infection for detection of M. agalactiae by culture were also negative. There were differences in the antigenic profiles of isolates recovered from the ears compared to the 7MAG strain used to inoculate the animals and most isolates from the mammary gland, milk and supramammary lymph nodes.

• 出版日期2011-10