Molecular prevalence and antibiotics resistance pattern of class A bla CTX-M-1 and bla TEM-1 beta lactamases in uropathogenic Escherichia coli isolates from Pakistan

作者：Rahman Hazir^*; Naeem Madiha; Khan Ilyas; Khan Jafar; Haroon Mohammad; Bari Fazli; Ullah Rahim; Qasim Muhammad

来源：Turkish Journal of Medical Sciences, 2016, 46(3): 897-902.

DOI：10.3906/sag-1502-14

• Urinary tract infection
• E. coli
• ESBLS
• bla CTX-M-1
• bla TEM-1
• antibiotics profile

Background/aim: Extended spectrum beta lactamase (ESBL) production among E. coli is one of the principal mechanisms that augment resistance to antibiotics. In the current study the molecular detection of class A beta lactamases among uropathogenic Escherichia coli was evaluated.
Materials and methods: A total of 355 urine samples were collected from a tertiary care hospital in Peshawar. The ESBL production among E. coli isolates was detected by using the disc synergy diffusion method. Moreover, the molecular detection of bla TEM-1 and bla CTX-M-1 ESBLs, the antibiotic resistance pattern, and the minimum inhibitory concentrations (MICs) were also documented.
Results: Among the 355 urine samples, 157 isolates were E. coli, and 23.56% of the isolates were ESBL E. coli. Among phenotypic ESBL producers, bla CTX-M-1 and bla TEM-1 were found in 59.45% and 40.54% of the isolates, respectively. A high resistance rate was observed against aztreonam (97.29%), while the lowest resistance was observed against imipenem (2.7%). The MICs of ESBLs E. coli for ceftriaxone, ciprofloxacin, and gentamicin was > 512 mu g/mL, 4 mu g/mL to 128 mu g/mL, and 1 mu g/mL to 14 mu g/mL respectively.
Conclusion: The present study showed that bla CTX-M-1 ESBL production is more prevalent in our clinical E. coli isolates. More often the ESBLs were resistant to commonly used antibiotics.

• 出版日期2016