Neutrophil extracellular traps in acute chorioamnionitis: Amechanism of host defense

作者：Gomez Lopez Nardhy^*; Romero Roberto^*; Leng Yaozhu; Garcia Flores Valeria; Xu Yi; Miller Derek; Hassan Sonia S

来源：American Journal of Reproductive Immunology, 2017, 77(3): UNSP e12617.

DOI：10.1111/aji.12617

• alarmins
• amniotic fluid
• DNA
• elastase
• infection
• inflammation
• parturition
• pregnancy
• preterm labor

ProblemNeutrophil extracellular traps (NETs) were recently described as a mechanism for microbial killing in the amniotic cavity of women with intra-amniotic infection. Such a clinical condition can result in acute chorioamnionitis, a placental lesion characterized by the infiltration of maternal neutrophils in the chorioamniotic membranes. Herein, we investigated whether these infiltrating neutrophils form NETs in the chorioamniotic membranes from women who underwent spontaneous term or preterm labor with acute chorioamnionitis.
Method of studyChorioamniotic membrane samples were collected from women who underwent spontaneous term or preterm labor with acute chorioamnionitis (n=10 each). Controls included chorioamniotic membrane samples from women who delivered at term or preterm with or without labor in the absence of acute chorioamnionitis (n=10 each). NETs were visualized and semiquantified in the chorioamniotic membranes by using antibodies against neutrophil elastase and histone H3 in combination with DAPI staining.
ResultsNeutrophil extracellular traps were abundant in the chorioamniotic membranes from women who underwent spontaneous term or preterm labor with acute chorioamnionitis. NETs were rarely found, or not visualized at all, in the chorioamniotic membranes from women who delivered at term or preterm with or without labor in the absence of acute chorioamnionitis.
ConclusionNeutrophil extracellular traps are abundant in the chorioamniotic membranes from women who underwent spontaneous term or preterm labor with acute chorioamnionitis. These findings suggest that chorioamniotic neutrophils can form NETs as a mechanism of host defense against infection or danger signals.

• 出版日期2017-3
• 单位NIH