Novel Components of an Active Mitochondrial K+/H+ Exchange

作者：Zotova Ludmila; Aleschko Markus; Sponder Gerhard^*; Baumgartner Roland; Reipert Siegfried; Prinz Monika; Schweyen Rudolf J; Nowikovsky Karin

来源：Journal of Biological Chemistry, 2010, 285(19): 14399-14414.

DOI：10.1074/jbc.M109.059956

Defects of the mitochondrial K+/H+ exchanger (KHE) result in increased matrix K+ content, swelling, and autophagic decay of the organelle. We have previously identified the yeast Mdm38 and its human homologue LETM1, the candidate gene for seizures in Wolf-Hirschhorn syndrome, as essential components of the KHE. In a genome-wide screen for multicopy suppressors of the pet(-) (reduced growth on nonfermentable substrate) phenotype of mdm38 Delta mutants, we now characterized the mitochondrial carriers PIC2 and MRS3 as moderate suppressors and MRS7 and YDL183c as strong suppressors. Like Mdm38p, Mrs7p and Ydl183cp are mitochondrial inner membrane proteins and constituents of similar to 500-kDa protein complexes. Triple mutant strains (mdm38 Delta mrs7 Delta ydl183c Delta) exhibit a remarkably stronger pet(-) phenotype than mdm38 Delta and a general growth reduction. They totally lack KHE activity, show a dramatic drop of mitochondrial membrane potential, and heavy fragmentation of mitochondria and vacuoles. Nigericin, an ionophore with KHE activity, fully restores growth of the triple mutant, indicating that loss of KHE activity is the underlying cause of its phenotype. Mdm38p or overexpression of Mrs7p, Ydl183cp, or LETM1 in the triple mutant rescues growth and KHE activity. ALETM1 human homologue, HCCR-1/LETMD1, described as an oncogene, partially suppresses the yeast triple mutant phenotype. Based on these results, we propose that Ydl183p and the Mdm38p homologues Mrs7p, LETM1, and HCCR-1 are involved in the formation of an active KHE system.

• 出版日期2010-5-7