A stearoyl-CoA-specific Delta 9 fatty acid desaturase from the basidiomycete Lentinula edodes

作者：Sakai H; Kajiwara S^*

来源：Bioscience Biotechnology and Biochemistry, 2003, 67(11): 2431-2437.

DOI：10.1271/bbb.67.2431

• fatty acid desaturation
• Delta 9 desaturase
• fruiting-body formation
• Lentinula edodes
• olel complementation

A gene encoding a Delta9 fatty acid desaturase (Delta9-desaturase) was isolated from L. edodes. The open reading frame of this gene (named Le-FAD1) consisted of 1416 bp. The predicted protein of 471 amino acids shares 47-57% sequence identity with Delta9-desaturases from other fungi. Expression of the Le-FAD1 ORF complemented genetic disruption of the Saccharomyces cerevisiae OLE1 gene (Sc-OLE1). Fatty acid analysis of the Le-FAD1-expressing S. cerevisiae ole1-disrupted strain showed that Le-FAD1 protein had slight activity with palmitic acid (16:0) as the substrate and high activity with stearic acid (18:0). Analysis of transcriptional expression showed that the levels of Le-FAD1 mRNA in the primordium and fruiting body of L. edodes were higher than in mycelia cultivated at 18degreesC as well as 25degreesC. These results correlated with the increase of unsaturated fatty acids (UFAs) in total lipids during fruiting-body formation.

• 出版日期2003-11