Low NDRG1 mRNA expression predicts a poor prognosis in neuroblastoma patients

作者：Matsushita Kohei; Uchida Keiichi^*; Saigusa Susumu; Ide Shozo; Hashimoto Kiyoshi; Koike Yuhki; Otake Kohei; Inoue Mikihiro; Tanaka Koji; Kusunoki Masato

来源：Pediatric Surgery International, 2013, 29(4): 363-368.

DOI：10.1007/s00383-012-3248-6

• Neuroblastoma
• NDRG1
• Prognostic marker
• Biomarker

N-myc downstream regulated gene 1 (NDRG1) markedly reduces metastasis of numerous tumors. However, NDRG1%26apos;s function in malignant tumors has not been fully determined. Therefore, we investigated the association of NDRG1 expression with clinical outcomes in neuroblastoma (NB) patients. %26lt;br%26gt;We obtained total RNA from residual cancer cells using microdissection from NB patients. Furthermore, we examined the expression of NDRG1 in NB patients using immunohistochemical staining. %26lt;br%26gt;Of the 48 patients observed, low NDRG1 expression was associated with poor prognostic factors such as primary tumor size and MYCN amplification. Low expression of NDRG1 was associated with a poor prognosis (p = 0.001) and multivariate analysis identified low expression of NDRG1 as an independent risk factor for predicting poor prognosis in NB patients. Furthermore, in the MYCN non-amplification group (n = 33), low expression of NDRG1 was associated with a poor prognosis (p = 0.001). Immunohistochemical analysis showed NDRG1 expression at the plasma membranes of NB cells. NDRG1 expression levels were also correlated with expression of NDRG1 mRNA. %26lt;br%26gt;We confirmed that low NDRG1 expression is a significant and independent prognostic indicator in NB by multivariate analysis. Furthermore, NDRG1 may be a novel prognostic marker in MYCN non-amplification NB patients.

• 出版日期2013-4