Appearance of high-molecular weight sialoglycoproteins recognized by Maackia amurensis leukoagglutinin in gastric cancer tissues: A case report using 2-DE-lectin binding analysis

作者：Inagaki Yoshinori; Usuda Mayumi; Xu Huanli; Wang Fengshan; Cui Shuxiang; Mafune Ken ichi; Sugawara Yasuhiko; Kokudo Norihiro; Tang Wei; Nakata Munehiro^*

来源：Bioscience Trends, 2008, 2(4): 151-154.

• Sialoglycoproteins
• Maackia amurensis leukoagglutinin
• Lectin
• 2-Dimensional electrophoresis
• Gastric cancer

Aberrant expression of sialoglycoconjugates has been thought to play an important role in cancer progression. Our previous lectin-histochemical study showed that overexpression of sialoglycoconjugates recognized by alpha 2,3-sialic acid-specific Maackia amurensis leukoagglutinin (MAL) was significantly related to the malignancy of gastric cancer. The present study analyzed the sialoglycoproteins in gastric cancer tissues by 2-dimensional electrophoresis (2-DE) in combination with lectin-binding analysis using MAL. Various MAL-positive sialoglycoproteins were detected in cancer tissues but not in non-cancer tissues. The sialoglycoproteins have a high molecular weight of near 200 kDa and over 200 kDa with different pI values for the two. This suggests that the MAL-positive sialoglycoproteins detected in gastric cancer tissues have high molecular weights and may contain different numbers of alpha 2,3-linked sialic acid residues in the carbohydrate moiety.

• 出版日期2008
• 单位山东省医学科学院; 山东大学