科研之友
微信
新浪微博
Facebook
分享链接
摘要
Objective: Splinting full-thickness cutaneous wounds in mice has allowed for a humanized model of wound healing. Delineating the epithelial edge and assessing time to closure of these healing wounds via macroscopic visualization have remained a challenge.
Approach: Double transgenic mice were created by crossbreeding K14-Cre and ROSA(mT/mG) reporter mice. Full-thickness excisional wounds were created in K14-Cre/ROSA(mT/mG) mice (n=5) and imaged using both normal and fluorescent light on the day of surgery, and every other postoperative day (POD) until wound healing was complete. Ten blinded observers analyzed a series of images from a single representative healing wound, taken using normal or fluorescent light, to decide the POD when healing was complete. K14-Cre/ROSA(mT/mG) mice (n=4) were subsequently sacrificed at the four potential days of rated wound closure to accurately determine the histological point of wound closure using microscopic fluorescence imaging.
Results: Average time to wound closure was rated significantly longer in the wound series images taken using normal light, compared with fluorescent light (mean POD 13.6 vs. 11.6, *p=0.008). Fluorescence imaging of histological samples indicated that reepithelialization was complete at 12 days postwounding.
Innovation: We describe a novel technique, using double transgenic mice K14-Cre/ROSA(mT/mG) and fluorescence imaging, to more accurately determine the healing time of wounds in mice upon macroscopic evaluation.
Conclusion: The accuracy by which wound healing can be macroscopically determined in vivo in mouse models of wound healing is significantly enhanced using K14-Cre/ROSA(mT/mG) double transgenic mice and fluorescence imaging.
- 出版日期2018-1
