Post-natal cardiomyocytes can generate iPS cells with an enhanced capacity toward cardiomyogenic re-differentation

作者：Rizzi R; Di Pasquale E; Portararo P; Papait R; Cattaneo P; Latronico M V G; Altomare C; Sala L; Zaza A; Hirsch E; Naldini L; Condorelli G^*; Bearzi C

来源：Cell Death and Differentiation, 2012, 19(7): 1162-1174.

DOI：10.1038/cdd.2011.205

• iPS cells
• cardiomyocytes
• cardiac fibroblasts
• cardiac repair

Adult mammalian cells can be reprogrammed to a pluripotent state by forcing the expression of a few embryonic transcription factors. The resulting induced pluripotent stem (iPS) cells can differentiate into cells of all three germ layers. It is well known that post-natal cardiomyocytes (CMs) lack the capacity to proliferate. Here, we report that neonatal CMs can be reprogrammed to generate iPS cells that express embryonic-specific markers and feature gene-expression profiles similar to those of mouse embryonic stem (mES) cell and cardiac fibroblast (CF)-derived iPS cell populations. CM-derived iPS cells are able to generate chimeric mice and, moreover, re-differentiate toward CMs more efficiently then either CF-derived iPS cells or mES cells. The increased differentiation capacity is possibly related to CM-derived iPS cells retaining an epigenetic memory of the phenotype of their founder cell. CM-derived iPS cells may thus lead to new information on differentiation processes underlying cardiac differentiation and proliferation. Cell Death and Differentiation (2012) 19, 1162-1174; doi: 10.1038/cdd.2011.205; published online 20 January 2012

• 出版日期2012-7