An efficient protocol for isolating melanised chaetothyrialean anamorphic fungi associated with plant-ants

作者:Ruiz Gonzalez Mario X*; Lauth Jeremie; Leroy Celine; Jauneau Alain; Gryta Herve; Jargeat Patricia; Dejean Alain; Orivel Jerome
来源:Journal of Basic Microbiology, 2013, 53(1): 98-100.
DOI:10.1002/jobm.201100539

摘要

Because of their ecological characteristics, slow growth rates and the presence of contaminants, Chaetothyriales fungi associated with structures built by tropical plant-ants can be difficult to isolate with standard procedures. Here, we describe an easy-to-use protocol for obtaining pure cultures by using cotton as a first substrate. We have further found by means of fluorescent stains that nuclei concentrate either in young hyphae or in the tips of the hyphae.

  • 出版日期2013-1