Development of Laser Ionization Techniques for Evaluation of the Effect of Cancer Drugs Using Imaging Mass Spectrometry

作者：Kannen Hiroki; Hazama Hisanao^*; Kaneda Yasufumi; Fujino Tatsuya; Awazu Kunio

来源：International Journal of Molecular Sciences, 2014, 15(7): 11234-11244.

DOI：10.3390/ijms150711234

• imaging mass spectrometry
• laser ionization
• anticancer drug
• drug resistance
• zeolite matrix

Recently, combined therapy using chemotherapy and photodynamic therapy (PDT) has been proposed as a means of improving treatment outcomes. In order to evaluate the efficacy of combined therapy, it is necessary to determine the distribution of the anticancer drug and the photosensitizer. We investigated the use of imaging mass spectrometry (IMS) to simultaneously observe the distributions of an anticancer drug and photosensitizer administered to cancer cells. In particular, we sought to increase the sensitivity of detection of the anticancer drug docetaxel and the photosensitizer protoporphyrin IX (PpIX) by optimizing the ionization-assisting reagents. When we used a matrix consisting of equal weights of a zeolite (NaY5.6) and a conventional organic matrix (6-aza-2-thiothymine) in matrix-assisted laser desorption/ionization, the signal intensity of the sodium-adducted ion of docetaxel (administered at 100 M) increased about 13-fold. Moreover, we detected docetaxel with the zeolite matrix using the droplet method, and detected PpIX by fluorescence and IMS with alpha-cyano-4-hydroxycinnamic acid (CHCA) using the spray method.

• 出版日期2014-7