Acetylated bovine serum albumin differentially inhibits polymerase chain reaction in microdevices

作者：Ramalingam Naveen^*; Warkiani Majid Ebrahimi; Gong Thomas Hai Qing^*

来源：Biomicrofluidics, 2017, 11(3): 034110.

DOI：10.1063/1.4983692

Bovine serum albumin (BSA) is widely used as an additive in polymerase chain reaction (PCR)-based microfluidic devices to passivate reactors and alleviate nucleic-acid amplification. BSA is available commercially in two types: either acetylated or non-acetylated. A survey of literature indicates that both types of BSA are used in PCR-based microfluidic devices. Our study results reveal that the use of acetylated BSA in PCR micro-devices leads to differential inhibition of PCR, compared to non-acetylated BSA. This result is noticed for the first time, and the differential inhibition generally goes un-noticed, as compared to complete PCR inhibition. Published by AIP Publishing.

• 出版日期2017-5
• 单位南阳理工学院