In Vitro Study on Cytotoxic Effects of ZnO Nanoparticles on Promastigote and Amastigote Forms of Leishmania major (MRHO/IR/75/ER)

作者：Delavari Mahdi; Dalimi Abdolhossein^*; Ghaffarifar Fatemeh; Sadraei Javid

来源：Iranian Journal of Parasitology, 2014, 9(1): 6-13.

• Leishmania major
• Amastigote
• Promastigote
• ZnO nanoparticles
• Apoptosis
• MTT assay

Background: Although pentavalent antimony compounds are used as antileishmanial drugs but they are associated with limitations and several adverse complications. Therefore, always effort to find a new and effective treatment is desired. In this study, the effect of ZnO nanoparticles with mean particle size of 20 nanometers (nm) on Leishmania major promastigotes and amastigotes was evaluated. %26lt;br%26gt;Methods: Viability percentage of promastigotes after adding different concentrations of ZnO nanoparticles (30, 60, 90 and 120 mu g/ml) to the parasite culture was evaluated by MTT assay. In the flow cytometry study, Annexin V-FITC Apoptosis detection Kit was used to study the induced apoptosis and necrotic effects. %26lt;br%26gt;Result: IC50 after 24 hours of incubation was 37.8 mu g/ml. ZnO nanoparticles exert cytotoxic effects on promastigotes of L. major through the induction of apoptosis. A concentration of 120 mu g/ml of ZnO nanoparticles induced 93.76% apoptosis in L. major after 72 hours. %26lt;br%26gt;Conclusion: ZnO NPs can induce apoptosis in L. major by dose and time-depended manner in vitro condition.

• 出版日期2014-3