Assessment of metabolic changes in the striatum of a MPTP-intoxicated canine model: in vivo H-1-MRS study of an animal model for Parkinson's disease

作者：Choi Chi Bong; Kim Sang Young; Lee Sung Ho; Jahng Geon Ho; Kim Hwi Yool; Choe Bo Young; Ryu Kyung Nam; Yang Dal Mo; Yim Sung Vin; Choi Woo Suk^*

来源：Magnetic Resonance Imaging, 2011, 29(1): 32-39.

DOI：10.1016/j.mri.2010.03.043

• Parkinson's disease
• Striatum
• 1-methyl 4-phenyl 1,2,3,6-tetrahydropyridine
• H-1-MRS
• Canine model

Parkinson's disease (PD) is a neurodegenerative disorder characterized by the progressive loss of the dopaminergic neurons in the substantia nigra pars compacta, which projects to the striatum. We induced a selective loss of nigrostriatal dopamine neurons, by infusing the mitochondrial complex 1 inhibitor 1-methyl 4-phenyl 1,2,3,6-tetrahydropyridine (MPTP) into adult beagle dogs (N=5). Single voxel H-1 water suppressed magnetic resonance spectroscopy (H-1-MRS) at 3 T was used to assess the metabolic changes in the striatum of canine before and after MPTP intoxication. The metabolite spectra obtained from the striatum (voxel size: 2 cm(3)) showed a lower N-acetyl aspartate to total creatine (creatine+phosphocreatine) ratio after MPTP intoxication. There were no significant differences in other metabolite ratios such as glutamate+glutamine, choline-containing compounds (glycerophosphocholine+phophorylcholine and myoinositol). Our findings indicated that H-1-MRS is a sensitive, noninvasive measure of neural toxicity and biochemical alterations of the striatum in a canine model of PD, and further studies are needed to confirm brain metabolic changes in association with progression of MPTP-intoxication.

• 出版日期2011-1