A sequence element that tunes Escherichia coli tRNA(GGC)(Ala) to ensure accurate decoding

作者：Ledoux Sarah; Olejniczak Mikolaj; Uhlenbeck Olke C^*

来源：Nature Structural & Molecular Biology, 2009, 16(4): 359-364.

DOI：10.1038/nsmb.1581

Mutating the rare A32-U38 nucleotide pair at the top of the anticodon loop of Escherichia coli tRNA(GGC)(Ala) to a more common U32-A38 pair results in a tRNA that performs almost normally on cognate codons but is unusually efficient in reading near-cognate codons. Pre-steady state kinetic measurements on E. coli ribosomes show that, unlike the wild-type tRNA(GGC)(Ala), the misreading mutant tRNA(GGC)(Ala) shows rapid GTP hydrolysis and no detectable proofreading on near-cognate codons. Similarly, tRNA(GGC)(Ala) mutated to contain C32-G38, a pair that is found in some bacterial tRNA(GGC)(Ala) sequences, was able to decode only the cognate codons, whereas tRNA(GGC)(Ala) containing a more common C32-A38 pair was able to decode all cognate and near-cognate codons tested. We propose that many of the phylogenetically conserved sequence elements present in each tRNA have evolved to suppress translation of near-cognate codons.

• 出版日期2009-4
• 单位西北大学