科研之友
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摘要
Dioscorea villoso (wild yam) rhizome extract is a medicinal herb that is commonly used to treat symptoms of menopause and rheumatoid arthritis. We had evidence from previous in vitro experiments that this extract is toxic and pro-fibrotic in renal cells and aimed to test whether this occurs in vivo. Sprague-Dawley rats received 0.79 g/kg/d D. villoso extract in their food or no treatment over 7, 14 and 28d (n = 4 per group). Kidney and liver tissues were collected for protein extraction and Western immunoblots or fixed for special histologic stains, immunohistochemistry (IHC) and microscopy. Collagen deposition was assessed using Masson's trichrome staining and morphometry. Macrophage infiltration (ED-1), epithelial-to-mesenchymal transdifferentiation or activation of fibroblasts (vimentin, alpha-SMA), and pro-fibrotic growth factors (TGF beta 1, CTGF) were assessed using IHC. Protein expression levels of the pro-inflammatory cytokine, TNF-alpha., the pro-fibrotic transcription factor, NF kappa B, a measure of oxidative stress (heme oxygenase-l), alpha-SMA, vimentin and TGF beta 1 were determined. Results showed that kidneys of the treated animals had significantly increased collagen, vimentin, TGF beta 1, NF kappa B, ED1, CTGF and alpha-SMA by 28d. In the liver. there was increased ED-1 and TGF beta 1 in the centrilobular zone at 28d in treated animals. In conclusion, there was no acute reno- or hepato-toxicity associated with administration of D. villoso. However, there was an increase in fibrosis in the kidneys and in inflammation in livers of rats consuming D. villosa for 28 days. Long term Supplementation with D. villoso may be best avoided, especially in people with compromised renal function and in those who need to take other drugs which may alter kidney function.
- 出版日期2008-9
