NLRC4 suppresses melanoma tumor progression independently of inflammasome activation

作者：Janowski Ann M; Colegio Oscar R; Hornick Emma E; McNiff Jennifer M; Martin Matthew D; Badovinac Vladimir P; Norian Lyse A; Zhang Weizhou; Cassel Suzanne L; Sutterwala Fayyaz S

来源：Journal of Clinical Investigation, 2016, 126(10): 3917-3928.

DOI：10.1172/JCI86953

Members of the NLR family can assemble inflammasome complexes with the adaptor protein ASC and caspase-1 that result in the activation of caspase-1 and the release of IL-1 beta and IL-18. Although the NLRC4 inflammasome is known to have a protective role in tumorigenesis, there is an increased appreciation for the inflammasome-independent actions of NLRC4. Here, we utilized a syngeneic subcutaneous murine model of B16F10 melanoma to explore the role of NLRC4 in tumor suppression. We found that NLRC4-deficient mice exhibited enhanced tumor growth that was independent of the inflammasome components ASC and caspase-1. Nlrc4 expression was critical for cytokine and chemokine production in tumor-associated macrophages and was necessary for the generation of protective IFN-gamma-producing CD4(+) and CD8(+) T cells. Tumor progression was diminished when WT or caspase-1-deficient, but not NLRC4-deficient, macrophages were coinjected with B16F10 tumor cells in NLRC4-deficient mice. Finally, examination of human primary melanomas revealed the extensive presence of NLRC4(+) tumor-associated macrophages. In contrast, there was a paucity of NLRC4(+) tumor-associated macrophages observed in human metastatic melanoma, supporting the concept that NLRC4 expression controls tumor growth. These results reveal a critical role for NLRC4 in suppressing tumor growth in an inflammasome-independent manner.

• 出版日期2016-10