A slide latex agglutination test for the rapid detection of antibodies in serum against porcine parvovirus

作者：Lu J; Zhao J; Fang L; He Q; Cao S; Chen H^*

来源：Journal of Veterinary Medicine Series B-Infectious Diseases and Veterinary Public Health, 2006, 53(2): 59-61.

DOI：10.1111/j.1439-0450.2006.00915.x

A slide latex agglutination test (LAT) was developed and evaluated to detect serum antibodies against porcine parvovirus. Porcine parvovirus antigen was obtained by 10% PEG-6000 and 0.5 mol/l sodium chloride precipitation, and inactivated by 0.1% methanal. Two per cent suspensions of latex particles (0.5-0.8 mu m) were coated by adding an equal volume of porcine parvovirus antigen at 0.34 mu g/ml. Repeatability of latex agglutination test was evaluated with a panel of 100 sera using the same and different antigen lots. A good agreement between LAT and haemagglutination inhibit assay was observed. Because of convenience and speed of performance, this method would be used widely in clinic examination.

• 出版日期2006-3
• 单位华中农业大学