Blood monocyte transcriptome and epigenome analyses reveal loci associated with human atherosclerosis

作者：Liu Yongmei^*; Reynolds Lindsay M; Ding Jingzhong; Hou Li; Lohman Kurt; Young Tracey; Cui Wei; Huang Zhiqing; Grenier Carole; Wan Ma; Stunnenberg Hendrik G; Siscovick David; Hou Lifang; Psaty Bruce M; Rich Stephen S; Rotter Jerome I; Kaufman Joel D; Burke Gregory L; Murphy Susan; Jacobs David R Jr; Post Wendy; Hoeschele Ina; Bell Douglas A; Herrington David; Parks John S; Tracy Russell P; McCall Charles E; Stein James H

来源：Nature Communications, 2017, 8(1): 393.

DOI：10.1038/s41467-017-00517-4

Little is known regarding the epigenetic basis of atherosclerosis. Here we present the CD14+ blood monocyte transcriptome and epigenome signatures associated with human atherosclerosis. The transcriptome signature includes transcription coactivator, ARID5B, which is known to form a chromatin derepressor complex with a histone H3K9Me2-specific demethylase and promote adipogenesis and smooth muscle development. ARID5B CpG (cg25953130) methylation is inversely associated with both ARID5B expression and atherosclerosis, consistent with this CpG residing in an ARID5B enhancer region, based on chromatin capture and histone marks data. Mediation analysis supports assumptions that ARID5B expression mediates effects of cg25953130 methylation and several cardiovascular disease risk factors on atherosclerotic burden. In lipopolysaccharide-stimulated human THP1 monocytes, ARID5B knockdown reduced expression of genes involved in atherosclerosis-related inflammatory and lipid metabolism pathways, and inhibited cell migration and phagocytosis. These data suggest that ARID5B expression, possibly regulated by an epigenetically controlled enhancer, promotes atherosclerosis by dysregulating immunometabolism towards a chronic inflammatory phenotype.

• 出版日期2017-8-30
• 单位UCLA; 美国弗吉尼亚理工大学(Virginia Tech); 西北大学; NIH