Binding of FKBP23 to BiP in ER shown by gel filtration chromatography

作者：Chen Chen; Ma Hui; Wang Ying; Mi Huaifeng^*

来源：Zeitschrift fur Naturforschung Section C-A Journal of Biosciences, 2007, 62(1-2): 133-137.

• FKBP23
• BiP
• gel filtration chromatography

FKBP23 was found in mouse endoplasmic reticulum (ER) in 1998. It consists of ark N-terminal peptidyl-prolyl cis/trans isomerase (PPIase) domain and a C-terminal domain with Ca2+ binding sites. Previously, we reported that FKBP23 specifically binds to BiP, the main protein of the molecular chaperone Hsp70 in ER lumen, and the binding is interrelated with the Ca2+ concentration. In this work we have found the existence of the complex FKBP23/BiP by separation of an ER extract using gel filtration chromatography (GFC), and that the existence of this complex is Ca2+-interrelated. This result further verified the Ca2+- interrelated binding of these two proteins in vivo.

• 出版日期2007-2
• 单位南开大学