Aptamer binding assays for proteins: The thrombin example-A review

作者：Deng Bin; Lin Yanwen; Wang Chuan; Li Feng; Wang Zhixin; Zhang Hongquan; Li Xing Fang; Le X Chris^*

来源：Analytica Chimica Acta, 2014, 837: 1-15.

DOI：10.1016/j.aca.2014.04.055

• Aptamers
• Affinity separation
• Binding-induced DNA assembly
• Catalytic amplification
• Homogeneous binding assays
• Thrombin

Experimentally selected single-stranded DNA and RNA aptamers are able to bind to specific target molecules with high affinity and specificity. Many analytical methods make use of affinity binding between the specific targets and their aptamers. In the development of these methods, thrombin is the most frequently used target molecule to demonstrate the proof-of-principle. This paper critically reviews more than one hundred assays that are based on aptamer binding to thrombin. This review focuses on homogeneous binding assays, electrochemical aptasensors, and affinity separation techniques. The emphasis of this review is placed on understanding the principles and unique features of the assays. The principles of most assays for thrombin are applicable to the determination of other molecular targets.

• 出版日期2014-7-21