A NOVEL APPROACH FOR STUDYING THE COMBINED ANTIMICROBIAL EFFECTS OF SILVER NANOPARTICLES AND ANTIBIOTICS THROUGH AGAR OVER LAYER METHOD AND DISK DIFFUSION METHOD

作者：Geoprincy G; Saravanan P; Gandhi N Nagendra; Renganathan S^*

来源：Digest Journal of Nanomaterials and Biostructures, 2011, 6(4): 1557-1565.

• Antibacterial activity
• Silver nanoparticles (AgNps)
• Antibiotics
• Thin layer chromatography (TLC)
• Bioautography
• Agar over layer method

In the present investigation, the combinatorial inhibitory effects of silver nanoparticles impregnated with four broad spectrum antibiotics namely amoxicillin, chloremphenicol, erythromycin and rifamycin against four major pathogens was analysed. For studying the antimicrobial activity, a technique based analysis was performed by disk diffusion method and by agar over layer method (Bioautography). It was observed that silver nanoparticles (10 mu g) showed potential zone of inhibition in similar with the effects of antibiotics (10 mu g). Rather, when given together (0.5 mu g each), an enhanced antibacterial activity depicting the inhibition zone (2 - 4 mm increase in diameter) was reported against certain pathogenic strains, Bacillus cereus (40 mm), Bacillus subtilis (28 mm), Klebsiella pneumoniea (24 mm) and Vibrio cholerae (20 mm). From this experimental analysis, rifamycin (antibiotic) offering maximum inhibitory effect, was separated by Thin Layer Chromatography (TLC). Bioautography (agar over layer method), a novel approach for studying the antimicrobial bahaviour of silver nanoparticles and silver nanoparticles impregnated with antibiotics was established. Two zones representing two reference points (black zone at loading point and red zone at separation point)) formed by agar over layer method indicates the zone of inhibtion produced by the silver nanoparticle and antibiotics respectively.

• 出版日期2011-12