A set of highly polymorphic microsatellites useful for kinship and population analysis in turbot (Scophthalmus maximus L.)

作者：Pardo Belen G; Hermida Miguel; Fernandez Carlos; Bouza Carmen; Perez Montse; Llavona Angela; Sanchez Laura; Martinez Paulino^*

来源：Aquaculture Research, 2006, 37(15): 1578-1582.

DOI：10.1111/j.1365-2109.2006.01600.x

• turbot
• microsatellites
• enriched library
• polymorphism
• kinship
• population analysis

The turbot (Scophthalmus maximus) is a flatfish species of great commercial value for aquaculture. In this study we describe the isolation and characterization of 30 novel highly polymorphic microsatellite markers in this species obtained from a genomic library enriched for seven short tandem repeated motifs. Much higher polymorphism (mean number of alleles: 13.37; mean expected heterozygosity: 0.869) and potential for parentage assignment than previously reported for microsatellites in turbot were found after the analysis of 24 wild individuals. Most loci conformed to Hardy-Weinberg expectations, excluding Sma-USC20 and Sma-USC28, which showed a high heterozygote deficit probably due to the presence of null alleles. No significant genotypic disequilibrium was observed between any pair of loci, suggesting no close linkage between them. These loci are potentially useful for kinship and population analysis in turbot.

• 出版日期2006-11-15