Dental monomers inhibit LPS-induced cytokine release from the macrophage cell line RAW264.7

作者：Bolling Anette Kocbach^*; Samuelsen Jan Tore; Mori**ak Else; Ansteinsson Vibeke; Becher Rune; Dahl Jon Einar; Mathisen Gro Haarklou

来源：Toxicology Letters, 2013, 216(2-3): 130-138.

DOI：10.1016/j.toxlet.2012.11.010

• Methacrylate monomers
• Cytokine
• Inflammation
• Combined exposure

Methacrylate monomers have been identified in aqueous extracts of freshly cured dental fillings. The hypothesis tested presently was that low concentrations of triethyleneglycol dimethacrylate (TEGDMA) and 2-hydroxyethyl methacrylate (HEMA) alone or in combination interfere with the LPS-induced release of cytokines from the macrophage cell line RAW264.7. The cells were exposed to 5-200 mu M of monomers for 24 h followed by a 24 h combined exposure to monomers and LPS. TEGDMA reduced LPS-induced release of interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha), whereas HEMA only reduced IL-1 beta release. Co-exposure to the two monomers indicated an additive effect. Moreover, the reduced cytokine release persisted for 24 h after termination of the monomer exposure. The LPS-induced activation of proteins in pre-transcriptional signaling pathways (CD14, p-ERK1/2, p-p38, p-JNK, p-I kappa B-alpha and p-NF kappa B-p65) was not altered by monomer exposure, neither were the levels of IL-1 beta and TNF-alpha mRNA. However, the LPS-induced level of pro-IL-1 beta was decreased by the monomer treatment. Thus, HEMA and TEGDMA may interfere with post-transcriptional regulation of synthesis and release of these cytokines. Overall, the results suggest that low concentrations of monomers may cause impaired macrophage responses, and that these effects can persist for up to 24 h after exposure.

• 出版日期2013-2-4