A method for transient expression in maize endosperm

作者：Requesens Deborah Vicuna^*; Egelkrout Erin; Devaiah Shivakumar; Hood Elizabeth E

来源：In Vitro Cellular and Developmental Biology - Plant, 2010, 46(6): 485-490.

DOI：10.1007/s11627-010-9305-8

• Zea mays
• Transient expression
• Endosperm
• Agrobacterium tumefaciens
• Cellulase

The ability to screen the functionality of gene constructs in a transient system of appropriate tissues informs the researcher of the potential success of stable transformation. For this purpose, we developed a transient system to test the functionality of endosperm-preferred promoters in maize. Two endosperm-preferred promoters from rice (a globulin and a glutelin promoter) were employed. Ears of Zea mays L. were harvested at 17 d after pollination, surface sterilized and the endosperm excised. Using Agrobacterium tumefaciens co-cultivation and sonication, transient expression of the target genes was detected after 4 and 5 d. We demonstrate expression of CBH I and CHB II (both exo-cellulases) up to 1.7% TSP, under the rice globulin and glutelin promoters.

• 出版日期2010-12