Iota-carrageenanichitosanigelatin scaffold for the osteogenic differentiation of adipose-derived MSCs in vitro

作者：Li Junjie; Yang Boguang; Qian Yufeng; Wang Qiyu; Han Ruijin; Hao Tong; Shu Yao; Zhang Yabin; Yao Fanglian; Wang Changyong^*

来源：Journal of Biomedical Materials Research Part B: Applied Biomaterials , 2015, 103(7): 1498-1510.

DOI：10.1002/jbm.b.33339

• carrageenan
• chitosan
• mesenchymal stem cells
• scaffold
• tissue engineering

In this study, we have developed u-carrageenan/ chitosan/gelatin (CCG) scaffold containing multiple functional groups (-NH2, -OH, -COOH, and SO3H) to resemble the native extracellular matrix (ECM), using the ionshielding technology and ultrasonic dispersion method. Fourier transform infrared spectroscopy (FTIR) of the CCG scaffolds suggests that the formation of CCG network involves electrostatic interactions between 1-carrageenan (ICA) and chitosan/gelatin, and the covalent cross-linking among amino groups of chitosan and/or gelatin. Scanning electron microscopic (SEMI observation reveals that the porous structure of scaffolds can be modulated by the ratio of t-CA to chitosan/gelatin. The swelling ratio of the hydrogels increases as the t-CA contents increase. Using differential scanning calorimetry, we found that the double helix structure of t-CA is only stabilized at low contents of t-CA in the CCG scaffolds (e.g., 5 wt /0). The scaffolds containing 5% t-CA showed the best protein adsorption capacity 14.46 0.63 pig protein/mg scaffold) and elastic modulus 15.37 1.03 MPa). In addition, the CCG scaffolds exhibit excellent support for adipose-derived mesenchymal stem cells (ADMSCs) attachment and proliferation, and they can improve the osteogenic differentiation and neovascularization capacities of ADMSCs. Overall, we conclude that the CCG may represent an ideal scaffold material for bone tissue engineering.

• 出版日期2015-10
• 单位中国人民解放军军事医学科学院; 天津大学