Mcl-1 Is Up Regulated by Prenylated Coumarin, Umbelliprenin in Jurkat Cells

作者：Gholami Omid; Jeddi Tehrani Mahmood; Iranshahi Mehrdad; Zarnani Amir Hassan; Ziai Seyed Ali^*

来源：Iranian Journal of Pharmaceutical Research, 2015, 14(4): 1387-1392.

• Umbellprenin
• Jurkat cell
• Apoptosis
• Mcl-1
• Real time PCR

Chronic lymphocytic leukaemia (CLL) is the most common B-cell malignancy in the western world and exists as subtypes with very different clinical courses. Myeloid cell leukemia 1 (Mcl-1) is one member of Bcl-2 family proteins that has been shown to be expressed in various tissues and malignant cells, including CLL, where its expression is significantly associated with a failure to achieve complete remission following cytotoxic therapy.
Induction of apoptosis by prenylated coumarin, umbelliprenin, in Jurkat cells was previously shown. We examined whether umbelliprenin can down-regulate Mcl-1 gene and protein in Jurkat cells. In this regard cells were incubated by umbelliprenin, and then down-regulation of Mcl-1 gene was studied by Real Time PCR method. Moreover, down-regulation of Mcl-1 protein was studied by western blot analysis.
We showed that, expression of Mcl-1 mRNA was increased from 1 hour to 3 hours incubation. but this increase has a scale down pattern. Moreover umbelliprenin could inhibit Mcl-1 protein. In conclusion umbelliprenin treatment modulates Mcl-1 expression at both the transcriptional and posttranslational levels.