Actinomycetes producers of beta-lactamases inhibitors with antimicrobial activity against microorganisms isolated from bovine mastitis

作者：Cunha M N C; Silva N M V; Teixeira M F S; Mota R A; Lima Filho J L; Porto T S; Porto A L F^*

来源：Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 2010, 62(6): 1312-1319.

DOI：10.1590/S0102-09352010000600003

• bovine mastitis
• beta-lactamase inhibitor
• actinomycetes
• antimicrobial activity
• Staphylococcus aureus

The ability of 71 actinomycetes, isolated from the Amazon lichens, to produce beta-lactamase inhibitors with antimicrobial activity was evaluated against penicillin-resistant Staphylococcus aureus, isolated from bovine mastitis in Pernambuco State. The selection of actinomycetes producers of beta-lactamase inhibitors was performed using agar-plug method against Klebsiella pneumoniae ATCC 29665 and the selected actinomycetes were tested against 17 penicillin-resistant Staphylococcus aureus strains. The best producers of beta-lactamase inhibitors were Streptomyces sp. DPUA 1542 and Nocardia sp. DPUA 1571. They were submitted to the submerged cultivation to determine the growth and pH curve, and antimicrobial activity. The highest inhibition halo zone was obtained by metabolites produced after 96 hours of cultivation for both Nocardia sp. (13.5 and 12.0mm) and Streptomyces sp. (8.0 and 14.0mm) with discs and well diffusion tests, respectively. The results showed that the actinomycetes are a promising source of beta-lactamase inhibitors, with potential for use in the bovine mastitis treatment.

• 出版日期2010-12