Apoptosis and cell cycle arrest of human colorectal cancer cell line HT-29 induced by vanillin

作者：Ho KetLi; Yazan Latifah Saiful; Ismail Norsharina; Ismail Maznah^*

来源：Cancer Epidemiology, 2009, 33(2): 155-160.

DOI：10.1016/j.canep.2009.06.003

• Cell viability
• MTS assay
• Cell morphology
• Acridine orange-ethidium bromide
• Cell cycle
• Apoptosis
• Annexin V-propidium iodide: 5-Bromo-2-deoxyuridine (BrdU): Anti-proliferation

Background: Vanillin is responsible for the flavor and smell of vanilla, a widely used flavoring agent. Previous studies showed that vanillin could enhance the repair of mutations and thus function as an anti-mutagen. However, its role in cancer, a disease that is closely related to mutation has not yet been fully elucidated. Methods: Hence, this study investigated the cytolytic and cytostatic properties of vanillin against HT-29, a human colorectal cancer cell line. Methods used including cell viability assay, acridine orange (AO)-ethidium bromide (EB) double staining cell morphological analysis, Cell cycle analysis, annexin V-propidium iodide apoptosis test and 5-bromo-2-deoxyuridine (BrdU)-labeling cell proliferation assay. Results: Results showed that apoptosis was induced by vanillin and the IC(50) for HT-29 and NIH/3T3 normal cell lines were 400 mu g/ml and 1000 mu g/ml, respectively. Different concentrations of vanillin arrest cell cycle at different checkpoints. 5-Bromo-2-deoxyuridine-labeling cell proliferation assay showed that G0/G1 arrest was achieved at lower concentration of vanillin (200 mu g/ml) while cell cycle analysis by flow cytometer showed that G2/M arrest occurs at higher concentration of vanillin (1000 mu g/ml). Conclusion: Cytolytic and cytostatic effects shown by vanillin showed that it could be a useful colorectal cancer preventive agent. Further in vivo study should be carried out to confirm that similar effects could happen in animals.

• 出版日期2009-8