alpha-Glucosidase inhibitory activity by the flower buds of Lonicera japonica Thunb

作者：Zhang, Zhong; Luo, Aimin; Zhong, Kai; Huang, Yina; Gao, Yuan; Zhang, Jun; Gao, Hong; Xu, Zhengjun; Gao, Xiaoling^*

来源：Journal of Functional Foods, 2013, 5(3): 1253-1259.

DOI：10.1016/j.jff.2013.04.008

• Lonicera japonica
• alpha-Glucosidase
• Maltase inhibitor
• Antidiabetes
• Polyphenols

In the present study, the methanol extract from the flower buds of Lonicera japonica Thunb. was investigated on rat intestinal alpha-glucosidase inhibitory activity. This extract enriched in polyphenols, showed a potent alpha-glucosidase inhibitory activity with maltose as a substrate in enzyme assay. The extract was then applied to MCI-gel column chromatography with a water-methanol gradient to give three fractions: fraction 1 (F-1, water), fraction 2 (F-2, 80% methanol in water), and fraction 3 (F-3, 100% methanol). F-2 showed the highest maltase inhibitory activity compared with F-1 and F-3, and down-regulated alpha-glucosidase activity for the maltose hydrolysis in Caco-2 monolayer. In maltose-loaded SD-rats, F-2 significantly reduced postprandial blood glucose level. 3,5-Dicaffeoylquinic acid was found to be the potent maltase inhibitor, whereas chlorogenic acid and rutin showed a weak inhibitory activity against maltase. This is the first report on the mammalian alpha-glucosdiase inhibition of L. japonica. The result presented here suggests a use of the extract of L. japonica for antidiabetes.

• 出版日期2013-7
• 单位西昌学院; 四川农业大学