Diversity of Clostridium difficile in pigs and other animals in Slovenia

作者：Avbersek Jana; Janezic Sandra; Pate Mateja; Rupnik Maja; Zidaric Valerija; Logar Katarina; Vengust Modest; Zemljic Mateja; Pirs Tina; Ocepek Matjaz^*

来源：Anaerobe, 2009, 15(6): 252-255.

DOI：10.1016/j.anaerobe.2009.07.004

• Clostridium difficile
• Animals
• PFGE
• Toxinotyping
• PCR-ribotyping

A study of Clostridium difficile diversity in pigs, calves and horses in Slovenia was conducted. A total of 547 samples were collected and C difficile was isolated from 247/485 (50.9%) piglet samples, from 4/42 (9.5%) calf samples, and 1/20 (5%) horse samples. The isolates were characterized by toxinotyping, PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) using restriction endonuclease Smal. Piglet isolates belonged to two toxinotypes (V and 0), four PCR-ribotypes (066, 029, SI 011, SI 010), and six pulsotypes. Bovine isolates were grouped into two toxinotypes (XIa and 0), three PCR-ribotypes (077, 002, 033), and three pulsotypes. The only equine isolate was indistinguishable from one calf isolate (XIa/033) in toxinotype, PCR-ribotype, and pulsotype. None of detected genotypes was present in all three animal hosts.

• 出版日期2009-12