Nitrifying granules cultivation in a sequencing batch reactor at a low organics-to-total nitrogen ratio in wastewater

作者：Cydzik Kwiatkowska A^*; Wojnowska Baryla I

来源：Folia Microbiologica, 2011, 56(3): 201-208.

DOI：10.1007/s12223-011-0037-x

It is possible to cultivate aerobic granular sludge at a low organic loading rate and organics-to-total nitrogen (COD/N) ratio in wastewater in the reactor with typical geometry (height/diameter = 2.1, superficial air velocity = 6 mm/s). The noted nitrification efficiency was very high (99%). At the highest applied ammonia load (0.3 +/- 0.002 mg NH (4) (+) -N g total suspended solids (TSS)(-1) day(-1), COD/N = 1), the dominating oxidized form of nitrogen was nitrite. Despite a constant aeration in the reactor, denitrification occurred in the structure of granules. Applied molecular techniques allowed the changes in the ammonia-oxidizing bacteria (AOB) community in granular sludge to be tracked. The major factor influencing AOB number and species composition was ammonia load. At the ammonia load of 0.3 +/- 0.002 mg NH (4) (+) -N g TSS(-1) day(-1), a highly diverse AOB community covering bacteria belonging to both the Nitrosospira and Nitrosomonas genera accounted for ca. 40% of the total bacteria in the biomass.

• 出版日期2011-5