Acid-Alkali Extraction of Triterpene Acids from Poria and Preparative Separation by High-Speed Counter-Current Chromatography

作者：Dong, Hongjing; Yan, Renyi; Li, Hua; Wang, Weihao; Wang, Xiao; Yang, Bin^*

来源：Separation Science and Technology (Philadelphia), 2014, 49(17): 2765-2771.

DOI：10.1080/01496395.2014.943769

• acid-alkali extraction
• preparative separation
• triterpene acids
• high-speed counter-current chromatography
• poria

A method for the acid-alkali extraction and preparative separation of triterpene acids from poria was established. The triterpene acids were enriched and separated into two fractions after extraction at the optimized pH value. The two fractions were subjected to high-speed counter-current chromatography for the preparative separation of triterpene acids, separately. As a result, dehydropachymic acid, pachymic acid, 3-epi-dehydropachymic acid, poricoic acid B, dehydrotumulosic acid, and 3-epi-dehydrotumulosic acid were obtained with purities of 94.1%, 96.2%, 93.5%, 85.9%, 80.1%, and 93.1%, respectively. The structures were identified by ESI-MS, H-1 NMR, and C-13 NMR.

• 出版日期2014-11-22
• 单位中国中医科学院; 山东省科学院