Daple Coordinates Planar Polarized Microtubule Dynamics in Ependymal Cells and Contributes to Hydrocephalus

作者：Takagishi Maki^*; Sawada Masato; Ohata Shinya; Asai Naoya; Enomoto Atsushi; Takahashi Kunihiko; Weng Liang; Ushida Kaori; Ara Hosne; Matsui Shigeyuki; Kaibuchi Kozo; Sawamoto Kazunobu; Takahashi Masahide^*

来源：Cell Reports, 2017, 20(4): 960-972.

DOI：10.1016/j.celrep.2017.06.089

Motile cilia in ependymal cells, which line the cerebral ventricles, exhibit a coordinated beating motion that drives directional cerebrospinal fluid (CSF) flow and guides neuroblast migration. At the apical cortex of these multi-ciliated cells, asymmetric localization of planar cell polarity (PCP) proteins is required for the planar polarization of microtubule dynamics, which coordinates cilia orientation. Daple is a disheveledassociating protein that controls the non-canonical Wnt signaling pathway and cell motility. Here, we show that Daple-deficient mice present hydrocephalus and their ependymal cilia lack coordinated orientation. Daple regulates microtubule dynamics at the anterior side of ependymal cells, which in turn orients the cilial basal bodies required for the directional cerebrospinal fluid flow. These results demonstrate an important role for Daple in planar polarity in motile cilia and provide a framework for understanding the mechanisms and functions of planar polarization in the ependymal cells.

• 出版日期2017-7-25