A colorimetric sandwich-type assay for sensitive thrombin detection based on enzyme-linked aptamer assay

作者：Park Jun Hee; Cho Yea Seul; Kang Sungmuk; Lee Eun Jeong; Lee Gwan Ho; Hah Sang Soo^*

来源：Analytical Biochemistry, 2014, 462: 10-12.

DOI：10.1016/j.ab.2014.05.015

• Aptamer
• Thrombin
• Signal amplification
• ELISA

A colorimetric sandwich-type assay based on enzyme-linked aptamer assay has been developed for the fast and sensitive detection of as low as 25 fM of thrombin with high linearity. Aptamer-immobilized glass was used to capture the target analyte, whereas a second aptamer, functionalized with horseradish peroxidase (HRP), was employed for the conventional 3,5,3%26apos;,5%26apos;-tetramethylbenzidine (TMB)-based colorimetric detection. Without the troublesome antibody requirement of the conventional enzyme-linked immunosorbent assay (ELISA), as low as 25 fM of thrombin could be rapidly and reproducibly detected. This assay has superior, or at least equal, recovery and accuracy to that of conventional antibody-based ELISA.

• 出版日期2014-10-1